Cellular Separation via Dielectrophoretic Field-Flow-Fractionation

I present a brief review of a cellular separation technique known as dielectrophoretic field-flowfractionation. The technique relies upon suspending different cells at different heights within a laminar flow profile. The differentially suspended cells, thus, travel at different speeds and elute from the flow chamber at different times resulting in successful separation. An alternate fabrication procedure than that commonly described in the literature is then proposed utilizing a micromolded polydimethylsiloxane elastomer cast over a thick photoresist. The proposed procedure decreases the number of steps in the fabrication meanwhile providing more precise control over the dimensions of the resultant device.